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The digestive system and food tests – GCSE Combined Science Biology

Foundation and Higher · Grade 4

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How food is broken down by enzymes and bile along the digestive system, and how to test food for starch, sugar, protein and fat.

Objectives

Specification references this topic covers: AQA 4.2.1, 4.2.2; Edexcel B1.10, B1.12.

The digestive system and food tests explained

Start here

Digestion is how your body breaks food down into pieces small enough to use. It happens along a long tube called the gut, with help from enzymes. Enzymes are chemicals that speed up reactions.

It matters because the food you eat is made of large molecules. Your blood cannot take those in. Until food is digested, you cannot use it to grow, repair your body or get energy.

By the end you will be able to follow food through the gut. You will name the main enzymes and what they do. You will also describe the food tests and what bile does.

Why we digest food

Starch, proteins and fats are large molecules. They are too big to pass through the wall of the gut into the blood. Think of trying to post a whole pizza through a letterbox. You need to cut it into small pieces first.

Digestion breaks the large molecules into small soluble molecules. Soluble means they dissolve. These small molecules can be absorbed into the blood.

The body uses them to build new carbohydrates, lipids and proteins. They can also be respired to release energy. Enzymes in the digestive system speed up this breakdown.

Following the food

Food starts in the mouth. The salivary glands add saliva, which contains an enzyme. Food then goes down the oesophagus, the tube that leads to the stomach.

The stomach churns the food. It also makes acid and protease, an enzyme that digests protein.

The liver, gall bladder and pancreas also add chemicals to the food. The liver makes bile, which is stored in the gall bladder. The pancreas makes enzymes that go into the small intestine.

The digestive system shows how living things are organised. Cells that are alike form a tissue. Different tissues form an organ, and several organs working together form an organ system. The stomach is an organ made of muscular tissue, which churns the food, glandular tissue, which makes digestive juices with enzymes and acid, and epithelial tissue, which covers the inside and the outside. The digestive system is the organ system that digests and absorbs food.

mouthoesophagusstomachsmallintestinelargeintestineanus
A flow chart of food passing from the mouth through the oesophagus, stomach, small intestine and large intestine to the anus.

The intestines

After the stomach, food passes into the small intestine. This is where most digestion happens. It is also where the soluble products of digestion are absorbed into the blood.

The small intestine is lined with villi, which are tiny folds. They give a large surface area, so more food can be absorbed quickly. They have thin walls and a rich supply of blood vessels.

What is left goes into the large intestine. Here water is absorbed from the undigested food.

The three main enzymes

Amylase breaks down starch into sugars. It is made in the salivary glands, the pancreas and the small intestine.

Protease breaks down proteins into amino acids. It is made in the stomach, the pancreas and the small intestine. Lipase breaks down lipids, which are fats and oils, into fatty acids and glycerol. It is made in the pancreas and the small intestine.

The ending of each name tells you the job. Amylase works on starch, protease on protein and lipase on lipids.

Bile

Bile is made in the liver and stored in the gall bladder. It is released into the small intestine. Bile is not an enzyme.

Bile is alkaline, so it neutralises the hydrochloric acid that comes from the stomach. This gives the right pH for the enzymes in the small intestine.

Bile also emulsifies fats. It breaks big drops of fat into many tiny droplets. This gives a larger surface area for lipase to work on, so the fat is digested faster.

Food tests

For starch, add iodine solution. It turns from orange-brown to blue-black. For sugar, add Benedict’s solution and heat it in a water bath. It turns from blue to green, yellow or brick-red.

For protein, add Biuret solution. It turns from blue to purple. For lipid, shake the food with ethanol, then pour the mixture into water. A cloudy white emulsion shows lipid is present.

First crush the food and mix it with distilled water. Wear eye protection, because the reagents can irritate the eyes.

Recap

Digestion breaks large molecules into small soluble ones. Most digestion and absorption happens in the small intestine.

Amylase: starch to sugars. Protease: protein to amino acids. Lipase: lipid to fatty acids and glycerol.

Bile neutralises acid and emulsifies fat. Iodine tests for starch, Benedict’s for sugar, Biuret for protein and ethanol for lipid.

Key points

Worked examples

Example 1

Name the enzyme that breaks down starch.

  1. The name of an enzyme ends in -ase.
  2. It matches the substrate, which is starch.
  3. The enzyme is amylase.

Answer: amylase

Common slips
  • Slip: protease Protease breaks down proteins.
  • Slip: lipase Lipase breaks down lipids.
  • Slip: bile Bile is not an enzyme.

Example 2

A student adds iodine to a food sample and it turns blue-black. Adds Benedict’s solution and heats it, and it stays blue. State what the food contains and what it does not contain.

  1. Blue-black with iodine means starch is present.
  2. Blue with Benedict’s means there is no sugar.
  3. The food contains starch but no sugar.

Answer: It contains starch but no sugar

Common slips
  • Slip: It contains sugar but no starch. The colours are the wrong way round.
  • Slip: It contains protein. The Biuret test was not done.
  • Slip: It contains starch and sugar. Benedict’s stayed blue, so there is no sugar.

Example 3

Explain how bile helps the digestion of fats.

  1. Bile emulsifies fat, which breaks large drops into small droplets.
  2. This gives a larger surface area for lipase to work on.
  3. Bile is also alkaline, so it neutralises acid from the stomach and gives the right pH for lipase.

Answer: Bile emulsifies fat to give a larger surface area for lipase and neutralises stomach acid

Common slips
  • Slip: Bile is an enzyme that digests fat. Bile is not an enzyme. Lipase digests fat.
  • Slip: Bile makes the fat drops bigger. Bile makes the drops smaller.
  • Slip: Bile is acidic. Bile is alkaline, which neutralises acid.

Questions and answers

The digestive system and food tests practice questions for GCSE Combined Science Biology. Try each one, then open the answer. Easy questions come first.

Question 1 Easy

Name the enzyme that digests protein.

Show answer

Answer: protease

  1. The name ends in -ase and matches protein.
  2. It is protease.

Question 2 Easy

Name the organ that makes bile.

Show answer

Answer: liver

  1. Bile is made in a large organ.
  2. It is made in the liver.

Question 3 Easy

Which reagent turns blue-black when starch is present?

Show answer

Answer: iodine

  1. Iodine is the test for starch.
  2. It turns blue-black.

Question 4 Easy

What are the products of lipid digestion? Give one.

Show answer

Answer: fatty acids

  1. Lipase breaks lipids down.
  2. The products are fatty acids and glycerol.

Question 5 Easy

Name the enzyme that breaks down lipids.

Show answer

Answer: lipase

  1. The ending -ase shows an enzyme.
  2. Lipids are broken down by lipase.

Question 6 Easy

Name the organ that absorbs water from undigested food.

Show answer

Answer: large intestine

  1. Water is taken back from what is left.
  2. This happens in the large intestine.

Question 7 Medium

Which colour does Biuret solution turn when protein is present?

Show answer

Answer: purple

  1. Biuret is the test for protein.
  2. It turns purple.

Question 8 Medium

Name the organ where bile is stored.

Show answer

Answer: gall bladder

  1. Bile is made in the liver.
  2. It is stored in the gall bladder.

Exam-style questions

Longer questions in the style of a written paper, with the marking points. These are Yumio practice questions.

Exam-style question 14 marks

(a) State the enzyme that breaks down lipids. [1 mark] (b) Name the two products. [1 mark] (c) Explain why bile makes this reaction faster. [2 marks]

Show answer and marking points

Answer: (a) lipase (b) fatty acids and glycerol (c) bile emulsifies fat into droplets, which gives a larger surface area for lipase to work on

  • (a) lipase (1)
  • (b) fatty acids and glycerol (1)
  • (c) bile emulsifies fat or breaks it into droplets (1)
  • (c) a larger surface area for lipase (1)

Exam-style question 24 marks

Describe how you would test a sample of food for the presence of protein and for a reducing sugar. [4 marks]

Show answer and marking points

Answer: Protein: add Biuret solution to the food solution. It turns purple if protein is present. Sugar: add Benedict’s solution and heat in a water bath. It turns green, yellow or brick-red if sugar is present.

  • protein: add Biuret solution (1)
  • purple shows protein (1)
  • sugar: add Benedict’s solution and heat in a water bath (1)
  • green, yellow or brick-red shows sugar (1)

Common mistakes

  1. Saying bile is an enzyme

    Bile is not an enzyme. It emulsifies fat and neutralises acid.

  2. Saying bile is made in the gall bladder

    Bile is made in the liver and stored in the gall bladder.

  3. Mixing up the colours in the food tests

    Learn them as a table: iodine blue-black, Benedict’s brick-red, Biuret purple, ethanol cloudy white.

  4. Saying digestion happens only in the stomach

    Most digestion and absorption happen in the small intestine.

Questions students ask

Is The digestive system and food tests on the Foundation or Higher tier?

The digestive system and food tests is on both Foundation and Higher papers for GCSE Combined Science Biology, aimed at around grade 4.

What is a common mistake on The digestive system and food tests?

Saying bile is an enzyme. Bile is not an enzyme. It emulsifies fat and neutralises acid.

Where are the answers to the The digestive system and food tests questions?

Open Show answer under each question on this page. The questions are Yumio practice questions, not past exam questions.

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