Active transport is how cells move useful substances into themselves, even when it looks like the wrong direction. The idea is simple. We will take it one small step at a time.
It matters because plants need minerals from the soil and your gut needs to take in glucose. Without active transport, a plant could not get the minerals it needs to grow properly.
By the end you will be able to say what active transport is and why it needs energy. You will give examples from plants and animals. You will also compare it with diffusion and osmosis.
Uphill, not downhill
Particles in a liquid spread out. In diffusion, they move from where there are lots of them to where there are fewer. This direction is called moving down a concentration gradient. A gradient is just a difference in concentration.
Active transport does the opposite. It moves particles from a low concentration to a high concentration. We say this is against the gradient. It is like pushing a bike up a hill. You have to use energy.
Rolling down the hill is easy and free. Pushing up the hill costs effort. That is the key difference.
Where the energy comes from
Cells get energy from respiration. Respiration is a chemical reaction that releases energy from glucose. Most of it happens in the mitochondria.
Mitochondria are tiny parts inside a cell. Think of them as the cell’s power stations. They release the energy that the cell then uses to do jobs.
Active transport is one of those jobs. If a cell cannot respire, it has no energy to spare for it. That is why a lack of oxygen, which respiration needs, can stop active transport.
How the carrier works
The cell membrane has carrier proteins built into it. A carrier protein is a protein that picks up one kind of particle. A particle sticks to the carrier on one side of the membrane.
The cell then uses energy to change the shape of the carrier. This pushes the particle through to the other side. The particle is released there, even though it was already crowded.
A cell that does a lot of active transport needs a lot of energy. So it has many mitochondria.
Active transport in plants
Soil water has only a small amount of mineral ions dissolved in it. Mineral ions are tiny charged particles that plants need, such as nitrate. A plant uses nitrate to make proteins.
Root hair cells are the cells on a root that reach out into the soil. The ions are more concentrated inside these cells than in the soil. So diffusion would move them the wrong way. The cells use active transport instead.
Waterlogged soil has little oxygen. Roots cannot respire well, so less active transport happens and the plant struggles to take in ions.
Active transport in animals
In your small intestine, digested food is absorbed into the blood. Right after a meal there is lots of glucose in the gut, so glucose moves into the blood by diffusion.
Later the glucose in the gut becomes lower than in the blood. Diffusion stops working. Active transport takes over and moves the glucose uphill, so that none is wasted.
A flow showing glucose moving from a low concentration in the gut to a higher concentration in the blood by active transport using energy.
Comparing the three processes
Diffusion moves any particles down a concentration gradient. It needs no energy. It is passive, which means the cell does no work.
Osmosis is the diffusion of water only, through a partially permeable membrane. It is also passive. Water moves from a dilute solution to a more concentrated solution.
Active transport moves particles against the gradient. It is the only one of the three that is active, because it uses energy from respiration. A good clue in a question is the direction. Low to high means active transport.
Recap
Active transport moves particles from a low concentration to a high concentration. This is against the gradient.
It uses carrier proteins and energy from respiration, released in mitochondria.
Root hair cells use it for mineral ions. The gut uses it for glucose.
Diffusion and osmosis are passive. Active transport is the only one that needs energy. If uptake stops without oxygen, think active transport.
Key points
Active transport moves substances against a concentration gradient.
It needs energy from respiration and uses carrier proteins.
Examples: mineral ions into root hair cells, glucose into the blood.
Diffusion and osmosis are passive. Active transport is the only one that needs energy.
Worked examples
Example 1
Name the process that uses energy to move substances from a low concentration to a high concentration.
Moving against a gradient needs energy.
The energy comes from respiration.
The process is active transport.
Answer: active transport
Common slips
Slip: diffusion Diffusion goes from high to low, with no energy.
Slip: osmosis Osmosis is only the movement of water.
Slip: respiration Respiration releases the energy. It is not the movement.
Example 2
Explain why root hair cells need to use active transport to take in nitrate ions.
The concentration of nitrate in the soil is lower than in the root hair cell.
Diffusion would move the nitrate out of the cell, down the gradient.
So the cell uses energy from respiration to take it in against the gradient.
Answer: The nitrate is at a lower concentration in the soil than in the cell, so active transport with energy from respiration moves it in
Common slips
Slip: The soil has a higher concentration of nitrate. The soil is the more dilute solution, so diffusion cannot be used.
Slip: Active transport lets water in. Water enters by osmosis. Active transport is for the ions.
Slip: It does not need energy. Moving against a gradient always needs energy.
Example 3
A farmer finds that crops in waterlogged soil grow poorly. Use your knowledge of active transport to explain why.
Waterlogged soil has little oxygen.
So the root cells cannot respire aerobically, and release less energy.
Less active transport of mineral ions happens, so the plant has less nitrate to make proteins and grows poorly.
Answer: Less oxygen means less respiration and energy, so less active transport of mineral ions and poor growth
Common slips
Slip: Water enters the root cells too quickly. The problem is the lack of oxygen for respiration.
Slip: Active transport does not need oxygen. It needs energy from respiration, which needs oxygen.
Slip: The mineral ions diffuse out of the roots. The effect is on energy supply, so less active transport happens.
Questions and answers
Active transport practice questions for GCSE Combined Science Biology. Try each one, then open the answer. Easy questions come first.
Question 1 Easy
Name the process that moves substances against a concentration gradient.
Show answer
Answer: active transport
The substance moves from low to high.
This needs energy and is active transport.
Question 2 Easy
Which process releases the energy used for active transport?
Show answer
Answer: respiration
Cells release energy from glucose.
This is respiration.
Question 3 Easy
Is osmosis an active or a passive process?
Show answer
Answer: passive
Osmosis needs no energy.
It is passive.
Question 4 Easy
Name the type of protein in the cell membrane that carries out active transport.
Show answer
Answer: carrier protein
The protein changes shape to move a particle.
It is a carrier protein.
Question 5 Easy
What name is given to the tiny parts of a cell that release energy by respiration?
Show answer
Answer: mitochondria
These are the power stations of the cell.
They are mitochondria.
Question 6 Easy
Does active transport move particles from low to high concentration, or from high to low?
Show answer
Answer: low to high
Active transport goes against the gradient.
So it goes from low to high.
Question 7 Medium
Name the cells in a plant that take in mineral ions by active transport.
Show answer
Answer: root hair cells
Roots absorb ions from the soil.
The cells are root hair cells.
Question 8 Medium
Give one mineral ion that plants take in by active transport.
Show answer
Answer: nitrate
Plants need nitrate to make proteins.
Nitrate is taken in by active transport.
Exam-style questions
Longer questions in the style of a written paper, with the marking points. These are Yumio practice questions.
Exam-style question 14 marks
(a) State what is meant by active transport. [2 marks] (b) Explain why a root hair cell contains many mitochondria. [2 marks]
Show answer and marking points
Answer: (a) movement of substances against a concentration gradient using energy from respiration (b) mitochondria release energy by respiration which is needed for active transport of mineral ions
(a) movement from a lower to a higher concentration, or against a concentration gradient (1)
(a) uses energy from respiration (1)
(b) mitochondria release energy by respiration (1)
(b) energy is needed for active transport of mineral ions into the root cell (1)
Exam-style question 24 marks
Compare diffusion and active transport. [4 marks]
Show answer and marking points
Answer: Diffusion moves particles from a high to a low concentration, down the gradient, but active transport moves them from low to high, against the gradient. Diffusion needs no energy, but active transport needs energy from respiration and uses carrier proteins.
diffusion is from high to low concentration, down the gradient (1)
active transport is from low to high concentration, against the gradient (1)
diffusion is passive and needs no energy (1)
active transport needs energy from respiration, using carrier proteins (1)
Common mistakes
Saying active transport goes from high to low concentration
Active transport goes from low to high, against the gradient.
Saying active transport uses no energy
Active transport needs energy from respiration.
Saying osmosis is active
Osmosis is passive. It is the diffusion of water.
Saying mitochondria make the energy for active transport
Mitochondria release the energy from glucose by respiration.
Questions students ask
Is Active transport on the Foundation or Higher tier?
Active transport is on both Foundation and Higher papers for GCSE Combined Science Biology, aimed at around grade 5.
What is a common mistake on Active transport?
Saying active transport goes from high to low concentration. Active transport goes from low to high, against the gradient.
Where are the answers to the Active transport questions?
Open Show answer under each question on this page. The questions are Yumio practice questions, not past exam questions.