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Microscopes and magnification: GCSE Combined Science Biology practice questions

Foundation and Higher · Grade 4

A second set of questions on microscopes and magnification. They are different from the ones on the topic page. Try each, then open the answer and compare your working.

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Question 1 Medium

Change 0.25 mm into micrometres.

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Answer: 250 µm

  1. Multiply mm by 1000 to get µm.
  2. 0.25 × 1000 = 250 µm.

Question 2 Medium

An image is 60 mm long and the magnification is ×200. Calculate the real size in mm.

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Answer: 0.3 mm

  1. real size = 60 ÷ 200.
  2. The real size is 0.3 mm.

Question 3 Medium

Change 3 mm into micrometres.

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Answer: 3000 µm

  1. 1 mm = 1000 µm.
  2. 3 × 1000 = 3000 µm.

Question 4 Medium

An image is 12 mm long and the real size is 0.5 mm. Calculate the magnification.

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Answer: ×24

  1. Magnification = image size ÷ real size.
  2. 12 ÷ 0.5 = 24.

Question 5 Hard

A drawing of a cell is 25 mm long. The real cell is 5 µm long. Calculate the magnification.

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Answer: ×5000

  1. 25 mm = 25 000 µm.
  2. 25 000 ÷ 5 = ×5000.

Question 6 Hard

A bacterium is 2 × 10⁻³ mm long. Give its length in micrometres.

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Answer: 2 µm

  1. 2 × 10⁻³ mm = 0.002 mm.
  2. 0.002 × 1000 = 2 µm.

Question 7 Hard

An image is 18 mm long and the magnification is ×900. Calculate the real size in micrometres.

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Answer: 20 µm

  1. 18 ÷ 900 = 0.02 mm.
  2. 0.02 × 1000 = 20 µm.

Question 8 Hard

Why can an electron microscope show the inside of a mitochondrion but a light microscope cannot? Give one word for the property.

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Answer: resolution

  1. The structures are very small.
  2. Only a higher resolution can separate them.

Quick checks

Short questions with options. Choose one before you open the answer.

Check 1

What is the equation for magnification?

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magnification = image size ÷ real size.

Check 2

What is resolution?

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The smallest distance between two points that can still be seen as separate.

Check 3

Why can an electron microscope show smaller structures than a light microscope?

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It has a higher resolution.

Check 4

Name two structures an electron microscope can show that a light microscope cannot.

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Ribosomes and the inside of mitochondria.

Check 5

Why is iodine added to a slide of onion cells?

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It stains the cells, so the nucleus and cell wall are easier to see.

Check 6

Why is a coverslip lowered at an angle?

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So that air bubbles are not trapped under it.

Check 7

Which objective lens do you start with on a microscope?

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The lowest-power lens.

Check 8

Which focus knob is used with a high-power lens?

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The fine focus knob.

Check 9

How many micrometres are in one millimetre?

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1000.

Check 10

How many nanometres are in one micrometre?

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1000.

Check 11

How do you change millimetres to micrometres?

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Multiply by 1000.

Check 12

How do you rearrange the magnification equation to find real size?

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real size = image size ÷ magnification.

Check 13

How do you rearrange the magnification equation to find image size?

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image size = real size × magnification.

Check 14

What is 0.002 mm in standard form?

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2 × 10⁻³ mm.

Check 15

Does magnification have a unit?

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No. It is written like ×200.

Check 16

Why must an electron microscope sample not be alive?

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It is viewed in a vacuum.

Check 17

What is magnification?

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How many times bigger the image looks than the real object.

Check 18

What does a light microscope use to form an image?

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Light and lenses.

Check 19

What does an electron microscope use to form an image?

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A beam of electrons.

Check 20

Why do you start with the lowest-power lens?

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It makes the specimen easier to find.

Check 21

What does the coarse focus knob do?

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It gives a rough focus. It is used with the low-power lens.

Check 22

How do you change micrometres to millimetres?

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Divide by 1000.

Check 23

What is standard form?

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A way to write very small or large numbers using powers of ten.

Check 24

What is a stain?

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A dye that makes parts of a cell easier to see.

Check 25

What is a scale bar?

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A line on a diagram that shows a real length.

Check 26

Why must both sizes be in the same unit in the magnification equation?

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Otherwise the answer is wrong, for example by a factor of 1000.

Check 27

Why estimate the size of a cell before calculating?

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To check that the final answer is a sensible size, within about the right power of ten.

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